MHC Class II I-A antibody

Catalog No. ABIN135545

The Mouse Monoclonal anti-MHC Class II I-A antibody has been validated for FACS. It is suitable to detect MHC Class II I-A in samples from Chicken.

Quick Overview for MHC Class II I-A antibody (ABIN135545)

Target: MHC Class II I-A

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II I-A antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: CIa

Product Details anti-MHC Class II I-A Antibody

Specificity: Chicken/Quail/Dove/Pigeon/Duck Ia, Mr 32 & 27 KDa

Characteristics: Mouse Anti-Chicken Ia-UNLB

Purification: Purified

Immunogen: Splenic leukocytes from an outbred nine-week-old chicken

Isotype: IgM

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro inhibition of MLR

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for MHC Class II I-A

Target: MHC Class II I-A

Alternative Name: Ia

Background: The chicken Ia antigen is expressed on B cells, a subpopulation of the monocyte/macrophage lineage of cells, and mitogen-activated T cells.