MHC Class II antibody

Catalog No. ABIN135567

Cited in 1 publication. This anti-MHC Class II antibody is a Mouse Monoclonal antibody detecting MHC Class II in FACS. Suitable for Chicken.

Quick Overview for MHC Class II antibody (ABIN135567)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 2G11

Product Details anti-MHC Class II Antibody

Specificity: Chicken/Pigeon/Caiman MHC Class II β-chain, Mr 30-42 kDa

Characteristics: Mouse Anti-Chicken MHC Class II-UNLB

Purification: Purified

Immunogen: Unknown

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , EM - Reported in literature , IP - Reported in literature , Purification - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-MHC Class II antibody (ABIN135567)

Mucksová, Plachý, Staněk, Hejnar, Kalina, Benešová, Trefil: "Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines." in: Veterinary research, Vol. 48, Issue 1, pp. 18, (2017) (PubMed).

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II

Background: The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).