MHC Class II antibody (PE)

Catalog No. ABIN135569

The Mouse Monoclonal anti-MHC Class II antibody has been validated for FACS. It is suitable to detect MHC Class II in samples from Chicken. There is 1 publication available.

Quick Overview for MHC Class II antibody (PE) (ABIN135569)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 2G11

Product Details anti-MHC Class II Antibody (PE)

Specificity: Chicken/Pigeon/Caiman MHC Class II β-chain, Mr 30-42 kDa

Characteristics: Mouse Anti-Chicken MHC Class II-PE

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Unknown

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , EM - Reported in literature , IP - Reported in literature , Purification - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-MHC Class II antibody (PE) (ABIN135569)

Weiss, Christensen, Zeuthen, Vogensen, Jakobsen, Frøkiær: "Lactobacilli and bifidobacteria induce differential interferon-β profiles in dendritic cells." in: Cytokine, Vol. 56, Issue 2, pp. 520-30, (2011) (PubMed).

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II

Background: The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).