FOXA2 antibody (AA 435-439)

Catalog No. ABIN1355837

The Rabbit Polyclonal anti-FOXA2 antibody has been validated for WB. It is suitable to detect FOXA2 in samples from Human.

Quick Overview for FOXA2 antibody (AA 435-439) (ABIN1355837)

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FOXA2 Antibody

Binding Specificity: AA 435-439

Specificity: The antibody detects endogenous level of total FoxA2 (HNF3b) protein.

Immunogen: Peptide sequence around AA 435-439 ( A-S-P-L-A) derived from Human FoxA2 (HNF3b) . Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: FoxA2(HNF3beta)

Background: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' . In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs, FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis, regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Molecular Weight: 50 kDa

NCBI Accession: NP_068556

UniProt: Q9Y261

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process