ChT antibody (Biotin)

Catalog No. ABIN135608

The Mouse Monoclonal anti-ChT antibody has been validated for FACS. It is suitable to detect ChT in samples from Chicken.

Quick Overview for ChT antibody (Biotin) (ABIN135608)

Target: ChT (High Affinity Choline Transporter (ChT))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This ChT antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: CT1

Product Details anti-ChT Antibody (Biotin)

Specificity: Chicken/Quail ChT1, Mr 63 & 103 kDa

Characteristics: Mouse Anti-Chicken ChT1-BIOT

Immunogen: Chicken thymocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro inhibition of T cell differentiation

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Alternative Name: ChT1

Background: ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90 % of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive. Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function. CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers. CT1 also recognizes quail cortical thymocytes.