Mouse anti-Human IgG1 (Hinge Region) Antibody (PE)

Catalog No. ABIN135627

Quick Overview for Mouse anti-Human IgG1 (Hinge Region) Antibody (PE) (ABIN135627)

Target: IgG1

Clonality: Monoclonal

Conjugate: PE

Application: ELISA

Clone: 4E3

Isotype: IgG1

Product Details

Binding Specificity: Hinge Region

Reactivity: Human

Host: Mouse

Specificity: Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA

Characteristics: Mouse Anti-Human IgG1 Hinge-PE

Purification: Protein A affinity column

Immunogen: Unknown

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Sep - Reported in literature , Purification - Reported in literature , Note - For direct immunostaining of IgG, positive cells in flow cytometry applications, clone HP, (SB Cat. No. ,) is recommended
• Working Dilutions: ELISA Purified (UNLB) antibody ≤ 5 g/mL AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use
Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Karsten, Mehta, Shin, Diefenbach, Slein, Karpinski, Irvine, Broge, Suscovich, Alter: "A versatile high-throughput assay to characterize antibody-mediated neutrophil phagocytosis." in: Journal of immunological methods, Vol. 471, pp. 46-56, (2019) (PubMed).

Violari, Cotton, Kuhn, Schramm, Paximadis, Loubser, Shalekoff, Da Costa Dias, Otwombe, Liberty, McIntyre, Babiker, Gibb, Tiemessen: "A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation." in: Nature communications, Vol. 10, Issue 1, pp. 412, (2019) (PubMed).

Boesch, Kappel, Mahan, Chu, Crowley, Osei-Owusu, Alter, Ackerman: "Enrichment of high affinity subclasses and glycoforms from serum-derived IgG using FcγRs as affinity ligands." in: Biotechnology and bioengineering, Vol. 115, Issue 5, pp. 1265-1278, (2019) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody