Rubisco antibody

Catalog No. ABIN1385063

The Rabbit Polyclonal anti-Rubisco antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect Rubisco in samples from Arabidopsis thaliana. There are 2+ publications available.

Quick Overview for Rubisco antibody (ABIN1385063)

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rubisco antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Rubisco Antibody

Cross-Reactivity: Human, Plant

Cross-Reactivity (Details): Arabidopsis thaliana,

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Arabidopsis thaliana RUBISCO

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Rubisco antibody (ABIN1385063)

Cheng, Fu, Liao, Xu, Zeng, Tang, Li, Lai, Yang: "Differential accumulation of specialized metabolite l-theanine in green and albino-induced yellow tea (Camellia sinensis) leaves." in: Food chemistry, Vol. 276, pp. 93-100, (2019) (PubMed).

Zhang, Chow, Liu, Zhang, Liu, Peng: "A magic red coat on the surface of young leaves: anthocyanins distributed in trichome layer protect Castanopsis fissa leaves from photoinhibition." in: Tree physiology, Vol. 36, Issue 10, pp. 1296-1306, (2016) (PubMed).

Target Details for Rubisco

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Alternative Name: RUBISCO

Background:

Synonyms: rbcL, RBL_SPIOL, Ribulose bisphosphate carboxylase large chain, RuBisCO large subunit, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.

Background: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.Sequence similarities: Belongs to the RuBisCO large chain family. Type I subfamily.Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Gene ID: 844754