CD8 antibody (AA 51-150)

Catalog No. ABIN1385272

This Rabbit Polyclonal antibody specifically detects CD8 in FACS, IHC (fro), WB, ELISA, IHC (p), IF (p) and IF (cc). It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for CD8 antibody (AA 51-150) (ABIN1385272)

Target: CD8

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-CD8 Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD8 antibody (ABIN1385272)

Zou, Guo, Lv, Huang, Li, Wang, Jiang, She: "Clinicopathologic implications of CD8+/Foxp3+ratio and miR-574-3p/PD-L1 axis in spinal chordoma patients." in: Cancer immunology, immunotherapy : CII, Vol. 67, Issue 2, pp. 209-224, (2017) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Mongkhoune, Xie, Wang, Chen, Zhou, Peng, He, Yu, Zhou, Luo, Zhao, Lu: "A constructed HLA-A2-restricted pMAGE-A1(278-286) tetramer detects specific cytotoxic T lymphocytes in tumour tissues in situ." in: The Journal of international medical research, Vol. 41, Issue 6, pp. 1811-24, (2013) (PubMed).

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background:

Synonyms: CD8, MAL, p32, Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a

Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gene ID: 925

UniProt: P01732