Osteocalcin antibody (AA 21-100)

Catalog No. ABIN1385851

This Rabbit Polyclonal antibody specifically detects Osteocalcin in WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc) and IF (p). It exhibits reactivity toward Human, Rat, Mouse and Dog and has been mentioned in 3+ publications.

Quick Overview for Osteocalcin antibody (AA 21-100) (ABIN1385851)

Target: Osteocalcin (BGLAP)

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteocalcin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Osteocalcin Antibody

Binding Specificity: AA 21-100

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Osteocalcin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Osteocalcin antibody (ABIN1385851)

Jiang, Zhou, Huang, Wu, Huang, Liang, Liu, Zheng, Zhao: "Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 6, pp. 2327-39, (2016) (PubMed).

Li, Zhou, Wang, Yang, Ding, Zhou: "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." in: Biomaterials, Vol. 37, pp. 218-29, (2015) (PubMed).

Ding, Li, Liu, Yang, Zhou, Zhou: "A nano-micro alternating multilayer scaffold loading with rBMSCs and BMP-2 for bone tissue engineering." in: Colloids and surfaces. B, Biointerfaces, Vol. 133, pp. 286-95, (2015) (PubMed).

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: Osteocalcin

Background:

Synonyms: Osteocalcin, Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein, BGLAP

Background: Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2 % of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.

Gene ID: 632

UniProt: P02818