DMO/Dmrt1a (AA 51-100) antibody

Catalog No. ABIN1386037

This Rabbit Polyclonal antibody specifically detects in WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) and ICC. It exhibits reactivity toward Human, Mouse, Rat and Sheepand has been mentioned in 1 publication.

Quick Overview for DMO/Dmrt1a (AA 51-100) antibody (ABIN1386037)

Target: DMO/Dmrt1a

Reactivity: Human, Mouse, Rat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 51-100

Cross-Reactivity: Human, Mouse, Rat, Sheep

Predicted Reactivity: Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Dmrt1a

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Li, Lu, Luo, Gao, Zhao, Ma: "Expression and cellular localization of double sex and mab-3 related transcription factor 1 in testes of postnatal Small-Tail Han sheep at different developmental stages." in: Gene, Vol. 642, pp. 467-473, (2017) (PubMed).

Target Details

Target: DMO/Dmrt1a

Background:

Synonyms: DMT1, CT154, Doublesex- and mab-3-related transcription factor 1, DM domain expressed in testis protein 1, DMRT1

Background: Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis (By similarity).

Gene ID: 1761

UniProt: Q9Y5R6