FICD antibody (AA 161-250)

Catalog No. ABIN1386319

This Rabbit Polyclonal antibody specifically detects FICD in WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) and ICC. It exhibits reactivity toward Mouse.

Quick Overview for FICD antibody (AA 161-250) (ABIN1386319)

Target: FICD (FIC Domain Containing (FICD))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FICD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-FICD Antibody

Binding Specificity: AA 161-250

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HYPE

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FICD

Target: FICD (FIC Domain Containing (FICD))

Alternative Name: HYPE/HIP13

Background:

Synonyms: Adenosine monophosphate-protein transferase FICD, AMPylator FICD, FIC domain containing, FIC domain containing protein, FIC domain-containing protein, Fic S phase protein cell division homolog, ficd, FICD_HUMAN, HIP-13, HIP13, Huntingtin interacting protein 13, Huntingtin interacting protein E, Huntingtin interactor protein E, Huntingtin yeast partner E, Huntingtin-interacting protein 13, Huntingtin-interacting protein E.

Background: Huntingtin yeast partner E is a 458 amino acid single-pass membrane protein. HYPE is thought to interact with Huntingtin, a protein which induces neurodegeneration when mutated. HYPE also contains two tetratricopeptide repeats (TPR), which may be involved in protein-protein interaction. The gene that encodes HYPE is located on chromosome 12, which encodes over 1,100 genes within 132 million bases and makes up about 4.5 % of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding C-type lectin proteins which mediate the NK cell response to MHC I interaction. Trisomy 12p leads to facial development defects, seizure disorders and a host of other symptoms varying in severity depending on the extent of mosaicism and is most severe in cases of complete trisomy.