CXCL12 antibody (AA 24-70)

Catalog No. ABIN1386329

The Rabbit Polyclonal anti-CXCL12 antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect CXCL12 in samples from Rat. There are 3+ publications available.

Quick Overview for CXCL12 antibody (AA 24-70) (ABIN1386329)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CXCL12 Antibody

Binding Specificity: AA 24-70

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Pig,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SDF-1/CXCL12

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CXCL12 antibody (ABIN1386329)

Wang, Chen, Tang, Jiang, Ji: "AMD3100 Attenuates Matrix Metalloprotease-3 and -9 Expressions and Prevents Cartilage Degradation in a Monosodium Iodo-Acetate-Induced Rat Model of Temporomandibular Osteoarthritis." in: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, Vol. 74, Issue 5, pp. 927.e1-927.e13, (2016) (PubMed).

Grootaert, da Costa Martins, Bitsch, Pintelon, De Meyer, Martinet, Schrijvers: "Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis." in: Autophagy, (2015) (PubMed).

Pei, Zeng, Han, Liao, Zhou, Li, Zhang, Liu, Zhang, Liu, Yao, Xu: "Renal interstitial infiltration and tertiary lymphoid organ neogenesis in IgA nephropathy." in: Clinical journal of the American Society of Nephrology : CJASN, Vol. 9, Issue 2, pp. 255-64, (2014) (PubMed).

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: SDF1

Background:

Synonyms: IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, hIRH, Pre-B cell growth-stimulating factor, CXCL12, SDF1A, SDF1B

Background: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development, required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.

Gene ID: 6387

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling