F4/80 antibody (AA 732-747)

Catalog No. ABIN1387314

This anti-F4/80 antibody is a Rabbit Polyclonal antibody detecting F4/80 in FACS, WB, IHC (p) and IF (p). Suitable for Mouse, Human and Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for F4/80 antibody (AA 732-747) (ABIN1387314)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-F4/80 Antibody

Binding Specificity: AA 732-747

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EMR1

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References for anti-F4/80 antibody (ABIN1387314)

Xue, Han, Ma, Wu, Qin, Liu, Hu, Hong, Hou: "Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo." in: BioMed research international, Vol. 2016, pp. 8916534, (2016) (PubMed).

Tsang, Huang, Yang, Chi, Chiang, Wang, Andocs, Szasz, Li, Chi: "Improving immunological tumor microenvironment using electro-hyperthermia followed by dendritic cell immunotherapy." in: BMC cancer, Vol. 15, pp. 708, (2015) (PubMed).

Kwan, Yeung, Liu, Wong, Shum, Lam, Cheng, Cheung, To: "Silver nanoparticles alter proteoglycan expression in the promotion of tendon repair." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 10, Issue 7, pp. 1375-83, (2014) (PubMed).

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: Emr1

Background:

Synonyms: TM7LN3, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, EMR1 hormone receptor, EMR1, Adhesion G Protein-Coupled Receptor E1, ADGRE1, Cell surface glycoprotein F4/80

Background: The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (13). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (13). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 Molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.

Gene ID: 2015

UniProt: Q14246