UBE2F antibody (AA 21-120)

Catalog No. ABIN1387600

This Rabbit Polyclonal antibody specifically detects UBE2F in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for UBE2F antibody (AA 21-120) (ABIN1387600)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UBE2F Antibody

Binding Specificity: AA 21-120

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NCE2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: NCE2

Background:

Synonyms: NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme, NEDD8 conjugating enzyme UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, NEDD8-conjugating enzyme UBE2F, ube2f, UBE2F_HUMAN, Ubiquitin conjugating enzyme E2F, Ubiquitin conjugating enzyme E2F putative, Ubiquitin-conjugating enzyme E2 F.

Background: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.