Progestin Receptor beta/mprb (AA 251-300) antibody

Catalog No. ABIN1387676

The Rabbit Polyclonal anti- antibody has been validated for ELISA, ICC, IF (cc), IF (p), IHC (fro), IHC (p) and WB. It is suitable to detect in samples from Dog, Mouse, Rat and Sheep. There is 1 publication available.

Quick Overview for Progestin Receptor beta/mprb (AA 251-300) antibody (ABIN1387676)

Target: Progestin Receptor beta/mprb

Reactivity: Dog, Mouse, Rat, Sheep

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Binding Specificity: AA 251-300

Cross-Reactivity: Dog, Mouse, Rat, Sheep

Predicted Reactivity: Human,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Progestin Receptor Beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Kasubuchi, Watanabe, Hirano, Inoue, Li, Terasawa, Konishi, Itoh, Kimura: "Membrane progesterone receptor beta (mPRβ/Paqr8) promotes progesterone-dependent neurite outgrowth in PC12 neuronal cells via non-G protein-coupled receptor (GPCR) signaling." in: Scientific reports, Vol. 7, Issue 1, pp. 5168, (2017) (PubMed).

Target Details

Target: Progestin Receptor beta/mprb

Background:

Synonyms: C6orf33, LMPB1, Lysosomal membrane protein in brain 1, Membrane progestin receptor beta, mPR beta, MPRB, PAQR8, Progestin and adipoQ receptor family member 8, Progestin and adipoQ receptor family member VIII, MPRB_HUMAN.

Background: The steroid progesterone induces the resumption of maturation in oocytes via a nongenomic pathway through binding to a novel membrane progestin receptor (mPR). This pathway inhibits adenylyl cyclase and reduces intracellular cAMP, and also activates mitogen-activated protein kinase to effect signal transduction pathways. Five distinct groups, designated Alpha, Beta, Gamma, Delta, comprise the mPR gene family. mPR Alpha, also designated progestin and adipo Theta receptor family member VII (PAQR7), consists of an extracellular N-terminus, an intracellular C-terminus and seven transmembrane domains. mPR Alpha is expressed in ovary, testis, placenta, uterus and bladder. mPR Beta, or progestin and adipo Theta receptor family member VIII (PAQR8), consists of eight putative transmembrane regions and an intracellular N-terminus that contains a leucine-rich motif. mPR Beta is a 354 amino acid protein expressed in brain and spinal cord. Both mPR Alpha and mPR Beta may be G protein-coupled receptors and may be involved in oocyte maturation.

Gene ID: 85315