UBE2U antibody (AA 171-270)

Catalog No. ABIN1388009

The Rabbit Polyclonal anti-UBE2U antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect UBE2U in samples from Human.

Quick Overview for UBE2U antibody (AA 171-270) (ABIN1388009)

Target: UBE2U (Ubiquitin-Conjugating Enzyme E2U (UBE2U))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2U antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2U Antibody

Binding Specificity: AA 171-270

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2U

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2U

Target: UBE2U (Ubiquitin-Conjugating Enzyme E2U (UBE2U))

Alternative Name: UBE2U

Background:

Synonyms: UBE2U, UBE2U_HUMAN, Ubiquitin carrier protein U, Ubiquitin-conjugating enzyme E2 U, Ubiquitin-protein ligase U.

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by a Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2U (ubiquitin-conjugating enzyme E2U) is a 321 amino acid E2 ubiquitin conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. Existing as two alternatively spliced isoforms, UBE2U is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8 % of the human genome.

Gene ID: 148581