BTD antibody (AA 401-500) (FITC)

Catalog No. ABIN1393478

The Rabbit Polyclonal anti-BTD antibody has been validated for IF (cc) and IF (p). It is suitable to detect BTD in samples from Rat.

Quick Overview for BTD antibody (AA 401-500) (FITC) (ABIN1393478)

Target: BTD (Biotinidase (BTD))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTD antibody is conjugated to FITC

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-BTD Antibody (FITC)

Binding Specificity: AA 401-500

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Biotinidase

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BTD

Target: BTD (Biotinidase (BTD))

Alternative Name: BTD/Biotinidase

Background:

Synonyms: Biotinase, Biotinidase, Btd, BTD_HUMAN, EC 3.5.1.12.

Background: Biotin, also known as vitamin B7, is an essential water-soluble vitamin that is a cofactor in glucogenesis and in the metabolism of fatty acids and leucine. Biotinidase is a 523 amino acid enzyme that catalyzes the hydrolysis of biocytin to biotin and lysine. Secreted into extracellular space, biotinidase is expressed in liver, heart, placenta, brain, skeletal muscle, pancreas and kidney. Biotinidase contains one carbon-nitrogen hydrolase domain, which is involved in the reduction of organic nitrogen compounds and ammonia production. Defects in the gene encoding biotinidase are the cause of biotinidase deficiency, which is characterized by skin rash, ataxia, seizures, hearing loss, hypotonia and optic atrophy. These symptoms are due to the individual?s inability to reutilize biotin and can, therefore, typically be treated with the addition of free biotin.