Acetyl-CoA Carboxylase alpha antibody (AbBy Fluor® 555)

Catalog No. ABIN1394057

Product Details anti-Acetyl-CoA Carboxylase alpha Antibody (AbBy Fluor® 555)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase alpha antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ACACA

Isotype: IgG

Application Details

Application Notes: IF(IHC-P)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Alternative Name: Acaca (ACACA Products)

Background:

Synonyms: ACAC, ACACA, ACACA, ACACA_HUMAN, ACC alpha, ACC-alpha, ACC1, ACC1, ACCA, Acetyl Coenzyme A, Biotin carboxylase, ACACA_MOUSE, Acetyl Coenzyme A carboxylase alpha, ACACA_RAT.

Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. ACC alpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCâ^ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACC alpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the enzyme’s short-term regulatory mechanism. The gene encoding ACC alpha maps to human chromosome 17 and encodes a form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCâ^ is homologous to that of the ACCå, except for an additional peptide of about 150 amino acids at the N-terminus.

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect