CD73 antibody (AA 151-250) (AbBy Fluor® 647)

Catalog No. ABIN1396721

The Rabbit Polyclonal anti-CD73 antibody has been validated for WB, FACS, IF (p) and IF (cc). It is suitable to detect CD73 in samples from Human, Mouse, Rat, Cow, Dog and Sheep. There is 1 publication available.

Quick Overview for CD73 antibody (AA 151-250) (AbBy Fluor® 647) (ABIN1396721)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-CD73 Antibody (AbBy Fluor® 647)

Binding Specificity: AA 151-250

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Predicted Reactivity: Cow,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD73/NT5

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD73 antibody (AbBy Fluor® 647) (ABIN1396721)

Ivanovska, Grolli, Borghetti, Ravanetti, Conti, De Angelis, Macchi, Ramoni, Martelli, Gazza, Cacchioli: "Immunophenotypical characterization of canine mesenchymal stem cells from perivisceral and subcutaneous adipose tissue by a species-specific panel of antibodies." in: Research in veterinary science, Vol. 114, pp. 51-58, (2017) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background:

Synonyms: NT, eN, NT5, NTE, eNT, CD73, E5NT, CALJA, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E

Background: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Gene ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process