BFSP2 antibody (AA 181-280) (HRP)

Catalog No. ABIN1406672

The Rabbit Polyclonal anti-BFSP2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect BFSP2 in samples from Human.

Quick Overview for BFSP2 antibody (AA 181-280) (HRP) (ABIN1406672)

Target: BFSP2 (Beaded Filament Structural Protein 2, Phakinin (BFSP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BFSP2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BFSP2 Antibody (HRP)

Binding Specificity: AA 181-280

Predicted Reactivity: Human,Mouse,Rat,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BFSP2/Phakinin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BFSP2

Target: BFSP2 (Beaded Filament Structural Protein 2, Phakinin (BFSP2))

Alternative Name: Bfsp2/Phakinin

Background:

Synonyms: 49 kDa cytoskeletal protein, Beaded filament protein CP49, Beaded filament structural protein 2, Beaded filament structural protein 2, phakinin, Bfps2, Cytoskeletal protein, 49 kD, BFSP2, BFSP2_HUMAN, CP47, CP49, Lens fiber cell beaded filament protein CP 47, Lens fiber cell beaded filament protein CP 49,Lens intermediate filament-like light, LL-L, Phakinin, PHAKOSIN.

Background: Phakinin is a membrane-associated and cytoskeletal intermediate filament (IF) protein specific to the eye lens. IFs are cytoskeletal structures that typically contain a head, rod and tail domain. Unlike most IFs, Phakinin completely lacks the C-terminal tail domain thus contributing to the unique structure of the beaded filament that is specific to the lens. Phakinin is required for the assembly of beaded filaments and cytoskeletal networks that are important for the long-term maintenance of optical properties and transparency of the lens. Phakinin copolymerizes with Filensin, another IF protein, to form the 10-nm filamentous structures of the beaded filaments. Phakinin is also capable of self-assembling into filament-like structures that form thicker bundles. Mutations in the gene encoding Phakinin can result in lens cataract.

Gene ID: 3921