Lamin B2 antibody (AA 61-160) (HRP)

Catalog No. ABIN1407356

The Rabbit Polyclonal anti-Lamin B2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Lamin B2 in samples from Mouse.

Quick Overview for Lamin B2 antibody (AA 61-160) (HRP) (ABIN1407356)

Target: Lamin B2 (LMNB2)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Lamin B2 Antibody (HRP)

Binding Specificity: AA 61-160

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Lamin B2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Alternative Name: Lamin B2

Background:

Synonyms: Alternative namesLAMB 2, LAMB2, Lamin-B2, LMN 2, LMN B2, LMN2, LMNB 2, LMNB2, LMNB2_HUMAN, MGC2721.

Background: A unique family of Cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed CED-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

Gene ID: 84823

UniProt: Q03252

Pathways: Apoptosis, Caspase Cascade in Apoptosis