SHANK2 antibody (AA 161-260) (Cy5)

Catalog No. ABIN1407982

This Rabbit Polyclonal antibody specifically detects SHANK2 in WB, IF (cc) and IF (p). It exhibits reactivity toward Mouse.

Quick Overview for SHANK2 antibody (AA 161-260) (Cy5) (ABIN1407982)

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHANK2 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SHANK2 Antibody (Cy5)

Binding Specificity: AA 161-260

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SHANK2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SHANK2

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Alternative Name: SHANK2

Background:

Synonyms: Cortactin binding protein 1, Cortactin SH3 domain-binding protein, Cortactin-binding protein 1, CortBP1, CTTNBP1, GKAP/SAPAP interacting protein, GKAP/SAPAP-interacting protein, KIAA1022, Proline rich synapse associated protein 1, Proline-rich synapse-associated protein 1, PROSAP1, SH3 and multiple ankyrin repeat domains protein 2, SHANK, Shank2, Shank2, SPANK3, SPANK-3, SHAN2_HUMAN.

Background: SH3 and multiple ankyrin repeat domains 1-3 (Shank1-3) of the Shank/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). The PSD is an electron-dense structure underneath the postsynaptic plasma membrane of excitatory synapses that anchors and clusters glutamate receptors opposite to the presynaptic neurotransmitter release site. Shank proteins contain PDZ modular domains that coordinate the synaptic localization of ion channels, receptors, signaling enzymes, and cell adhesion molecules. The PDZ domain mediates protein-protein interactions via the recognition of a conserved sequence motif at the C-terminus of their target protein(s). Shank recruits betaPIX and PAK to spines to regulate postsynaptic structure and interacts with NMDA receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.