Hsp 40-4/Hdj2/Dnaja1 (AA 21-110) antibody (HRP)

Catalog No. ABIN1408208

The Rabbit Polyclonal anti- antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect in samples from Mouse.

Quick Overview for Hsp 40-4/Hdj2/Dnaja1 (AA 21-110) antibody (HRP) (ABIN1408208)

Target: Hsp 40-4/Hdj2/Dnaja1

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 21-110

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HSP 40-4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Hsp 40-4/Hdj2/Dnaja1

Background:

Synonyms: DJ 2, DJ2, DjA1, DnaJ Hsp40 homolog, subfamily A, member 1, DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, DNAJ2, Dnaja1, DNJA1_HUMAN, hDJ 2, HDJ-2, HDJ2, Heat shock 40 kDa protein 4, heat shock protein DNAJ like 2, Heat shock protein J2, HSDJ, HSJ-2, HSJ2, HSPF4, Human DnaJ protein 2, NEDD7, Neural precursor cell expressed developmentally down regulated 7, OTTHUMP00000021193.

Background: DnaJ-like proteins interact with HSP 70 Molecular chaperones and function to facilitate protein folding and mitochondrial protein import. HSP 40-4, also known as HDJ2, is the human DnaJ homolog that functions as a co-chaperone with a cysteine-rich zinc finger domain. The cellular redox enzyme thioredoxin interacts with HSP 40-4, and oxidation and reduction reversibly regulate HSP 40-4 function in response to the changing redox states of the cell. The zinc finger domain of HSP 40-4 may act as a redox sensor of chaperone-mediated protein-folding machinery, since HSP 40-4 inactivation leads to the oxidation of cysteine thiols and a simultaneous release of coordinated zinc. Loss of the HSP 40-4 protein may be linked to severe defects in spermatogenesis that involve aberrant androgen signaling.