PCYT1A antibody (AA 201-300) (Cy5.5)

Catalog No. ABIN1408379

The Rabbit Polyclonal anti-PCYT1A antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect PCYT1A in samples from Human and Mouse.

Quick Overview for PCYT1A antibody (AA 201-300) (Cy5.5) (ABIN1408379)

Target: PCYT1A (Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCYT1A antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PCYT1A Antibody (Cy5.5)

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCYT1A

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PCYT1A

Target: PCYT1A (Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A))

Alternative Name: PCYT1A

Background:

Synonyms: CCT A, CCT alpha, CCT-alpha, Choline phosphate cytidylyltransferase A, Choline-phosphate cytidylyltransferase A, CT A, CT antibody CTP:phosphocholine cytidylyltransferase A, CTPCT, PCY1A_HUMAN, PCYT1, Pcyt1a, Phosphate cytidylyltransferase 1 choline alpha, Phosphate cytidylyltransferase 1 choline alpha isoform, Phosphorylcholine transferase A.

Background: Increase in fetal surfactant synthesis and lung maturity is caused by the glucocorticoidal induction of enzymes required for phosphatidylcholine synthesis towards the end of gestation (1). The regulation of gestational age-dependent induction of phosphatidylcholine synthesis by glucocorticoids is still unclear (1). The rate-controlling enzyme in the phosphatidylcholine biosynthetic pathway is CTP-phosphocholine cytidylyltransferase A (CCT A) (24). In cultured eukaryotic cells, this enzyme is essential for survival (3). The alpha isoform is located in the nucleus and is regulated by reversible phosphorylation and membrane association (3). There is significant identity between the alpha-helical membrane-binding domains of CCT A and soybean oleosin (2). Expressed CCT A has lipid-dependent cytidylyltransferase activity (5). The gene which encodes CCT A maps to human chromosome 3q (4).