ZFYVE27 antibody (AA 341-411) (Cy5.5)

Catalog No. ABIN1411151

The Rabbit Polyclonal anti-ZFYVE27 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect ZFYVE27 in samples from Human, Rat and Mouse.

Quick Overview for ZFYVE27 antibody (AA 341-411) (Cy5.5) (ABIN1411151)

Target: ZFYVE27 (Zinc Finger, FYVE Domain Containing 27 (ZFYVE27))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZFYVE27 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ZFYVE27 Antibody (Cy5.5)

Binding Specificity: AA 341-411

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ZFYVE27

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ZFYVE27

Target: ZFYVE27 (Zinc Finger, FYVE Domain Containing 27 (ZFYVE27))

Alternative Name: ZFYVE27

Background:

Synonyms: Protrudin, RP11 459F3.2, SPG33, ZFY27_HUMAN, ZFYVE27, zinc finger FYVE domain containing 27, Zinc finger FYVE domain containing protein 27, Zinc finger FYVE domain-containing protein 27.

Background: Zinc finger FYVE domain-containing protein 27 (ZFYVE27), also known as SPG33, is a 411 amino acid member of the FYVE-finger family of proteins. The FYVE domain is a cysteine-rich domain of about 70 amino acids that plays a role in the endosomal localization of the FYVE-finger proteins, and a majority of these proteins serve as regulators of endocytic membrane trafficking. ZFYVE27, a multi-pass membrane protein, is an endosomal protein that binds to Spastin, a protein that is primarily involved in microtubule dynamics and severing, vesicular trafficking and endosomal trafficking. Mutations in the gene encoding ZFTVE27 affect neuronal intracellular trafficking in the corticospinal tract and are thought to lead to hereditary spastic paraplegia (HSP), a neurodegenerative disorder, characterized by progressive paralysis of the legs, which is caused by impaired axonal transport. Five isoforms of ZFYVE27 exist as a result of alternative splicing events.

Gene ID: 118813

UniProt: Q5T4F4

Pathways: Neurotrophin Signaling Pathway