DDAH1 antibody (AA 201-285) (HRP)

Catalog No. ABIN1412450

The Rabbit Polyclonal anti-DDAH1 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect DDAH1 in samples from Mouse.

Quick Overview for DDAH1 antibody (AA 201-285) (HRP) (ABIN1412450)

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDAH1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DDAH1 Antibody (HRP)

Binding Specificity: AA 201-285

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DDAH1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DDAH1

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Alternative Name: DDAH1

Background:

Synonyms: DDAH, DDAH I, DDAH-1, DDAH1, DDAH1_HUMAN, DDAHI, Dimethylargininase 1, Dimethylargininase-1, Dimethylarginine dimethylaminohydrolase 1, NG, NG-dimethylarginine dimethylaminohydrolase 1, NG NG dimethylarginine dimethylaminohydrolase.

Background: DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production (1,2). Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation (3). DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA) (4). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues (1).

Gene ID: 23576

UniProt: O94760