DDX3X antibody (pThr322) (HRP)

Catalog No. ABIN1413578

The Rabbit Polyclonal anti-DDX3X antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect DDX3X in samples from Human.

Quick Overview for DDX3X antibody (pThr322) (HRP) (ABIN1413578)

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3X antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DDX3X Antibody (HRP)

Binding Specificity: pThr322

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human DDX3 around the phosphorylation site of Thr322

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Alternative Name: DDX3

Background:

Synonyms: p-DDX3 phospho T322, ATP dependent RNA helicase DDX3X, ATP-dependent RNA helicase DDX3X, CAP Rf, DBX, DDX14, DDX3X, DDX3X_HUMAN, DEAD Asp Glu Ala Asp box polypeptide 3 X linked, DEAD box, DEAD box protein 3, DEAD box protein 3 X-chromosomal, DEAD box X isoform, DEAD/H Asp Glu Ala Asp/His box polypeptide 3, DEAD/H box 3, Fibroblast Growth Factor Inducible 14, Fin14, Helicase like protein 2, Helicase-like protein 2, HLP2, X isoform, X-chromosomal.

Background: DDX3 is involved in RNA metabolism. Two DDX3 paralogs are found in humans, DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections.

Gene ID: 1654

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly