CH25H antibody (AA 1-100) (HRP)

Catalog No. ABIN1415409

This anti-CH25H antibody is a Rabbit Polyclonal antibody detecting CH25H in WB. Suitable for Mouse.

Quick Overview for CH25H antibody (AA 1-100) (HRP) (ABIN1415409)

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CH25H antibody is conjugated to HRP

Application: Western Blotting (WB)

Product Details anti-CH25H Antibody (HRP)

Binding Specificity: AA 1-100

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CH25H

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Alternative Name: CH25H

Background:

Synonyms: C25H, Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, CH25H

Background: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Gene ID: 9023

UniProt: O95992