MID1 antibody (AA 200-250) (Cy5.5)

Catalog No. ABIN1420255

This Rabbit Polyclonal antibody specifically detects MID1 in WB and IF (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MID1 antibody (AA 200-250) (Cy5.5) (ABIN1420255)

Target: MID1 (Midline 1 (MID1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MID1 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MID1 Antibody (Cy5.5)

Binding Specificity: AA 200-250

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Midline-1/RNF59

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MID1

Target: MID1 (Midline 1 (MID1))

Alternative Name: Midline-1

Background:

Synonyms: BBBG1, Finger on X and Y mouse homolog of antibody, FXY, GBBB1, MID-1, Mid1, Midin, Midline 1 Opitz/BBB syndrome, Midline 1, Midline 1 ring finger, Midline 1 RING finger protein, Midline-1, Midline1, OGS1, OSX, Putative transcription factor XPRF, RING finger protein 59, RNF59, TRI18, TRI18_HUMAN, TRIM18, Tripartite mot containing protein 18, Tripartite mot protein TRIM18, Tripartite mot-containing protein 18, XPRF, Zinc finger X and Y antibody, ZNFXY.

Background: Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1. Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Defects in MID1 are the cause of Opitz syndrome type I (OS-I). OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.

Gene ID: 4281

UniProt: O15344