SCIMP antibody

Catalog No. ABIN1449307

This Mouse Monoclonal antibody specifically detects SCIMP in IP, WB, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for SCIMP antibody (ABIN1449307)

Target: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCIMP antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: NVL-07

Product Details anti-SCIMP Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A affinity chromatography

Purity: > 95 % pure by SDS-PAGE

Immunogen: Recombinant intracellular part of human SCIMP

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), approx. pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SCIMP

Target: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Alternative Name: SCIMP

Background: SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.Synonyms: SLP adaptor and Csk interacting membrane protein

Gene ID: 388325

NCBI Accession: NP_996986

UniProt: Q6UWF3