AKTIP antibody (Middle Region)

Catalog No. ABIN1449900

This anti-AKTIP antibody is a Rabbit Polyclonal antibody detecting AKTIP in WB. Suitable for Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Chicken and Xenopus laevis.

Quick Overview for AKTIP antibody (Middle Region) (ABIN1449900)

Target: AKTIP (AKT Interacting Protein (AKTIP))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKTIP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKTIP Antibody

Binding Specificity: Middle Region

Sequence: NPSVHDEARE KMLTQKKPEE QHNKSVHVAG LSWVKPGSVQ PFSKEEKTVA

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Chicken, Dog, African clawed frog, ZebrafishSpecies reactivity (tested):Human

Purification: Purified using peptide immunoaffinity column

Immunogen: Synthetic peptide directed towards the middle region of human AKTIP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Add 50 μL of distilled water to a final concentration of 1 mg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C or at -20 °C long term. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or in aliquots at -20 °C long term.

Target Details for AKTIP

Target: AKTIP (AKT Interacting Protein (AKTIP))

Alternative Name: AKTIP / FTS

Background: AKTIP is the component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). AKTIP regulates apoptosis by enhancing phosphorylation and activation of AKT1. AKTIP increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade.The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.Synonyms: AKT-interacting protein, FT1, Fused toes protein homolog

Gene ID: 64400

NCBI Accession: NP_001012398