PITX3 antibody (N-Term)

Catalog No. ABIN1495362

This anti-PITX3 antibody is a Rabbit Polyclonal antibody detecting PITX3 in IHC, IHC (p) and IF. Suitable for Human and Mouse.

Quick Overview for PITX3 antibody (N-Term) (ABIN1495362)

Target: PITX3 (Pituitary Homeobox 3 (PITX3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PITX3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-PITX3 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Recognizes mouse PiTX3 protein. Reactivity has been confirmed with fetal mouse brain homogenate.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the N-terminal region of the mouse PiTX3 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (0.7 - 2 μg/mL), IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for 1 year.

Target Details for PITX3

Target: PITX3 (Pituitary Homeobox 3 (PITX3))

Alternative Name: PITX3 / PTX3

Background: Name/Gene ID: PITX3

Synonyms: PITX3, CTPP4, Homeobox protein PITX3, Paired-like homeodomain 3, Pituitary homeobox 3

Gene ID: 5309

UniProt: O75364