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Digoxigenin antibody (Alkaline Phosphatase (AP))

This Mouse Monoclonal antibody specifically detects Digoxigenin in ELISA, WB, IHC, ISH and SB. It exhibits reactivity toward Chemical.
Catalog No. ABIN1514935

Quick Overview for Digoxigenin antibody (Alkaline Phosphatase (AP)) (ABIN1514935)

Target

Digoxigenin (DIG)

Reactivity

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Chemical

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Digoxigenin antibody is conjugated to Alkaline Phosphatase (AP)

Application

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ELISA, Western Blotting (WB), Immunohistochemistry (IHC), In situ hybridization (ISH), Southern Blotting (SB)

Clone

21H8
  • Specificity

    Reacts with free and bound digoxigenin

    Purification

    Purified (protein A)

    Immunogen

    Digoxigenin

    Isotype

    IgG1 kappa
  • Application Notes

    Working dilution: Optimal dilution should be determined by the end user.
    The following are guidelines only:
    - IHC : 1/200 to 1/2000 - ELISA : 1/500 to 1/5000 - WB : 1/500 to 1/5000 - SB : 1/500 to 1/5000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.2 mg/mL

    Buffer

    Tris 10 mM NaCl 0.14M,MgCl2/ZnCl2 1 mM, Glycerol 50 % , Thiomersal 0.02 %

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    Digoxigenin (DIG)

    Alternative Name

    Digoxigenin

    Target Type

    Chemical

    Background

    Digoxigenin (DIG) is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications.
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