SP1 antibody (pThr453)

Catalog No. ABIN1531394

This anti-SP1 antibody is a Rabbit Polyclonal antibody detecting SP1 in WB, ELISA, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for SP1 antibody (pThr453) (ABIN1531394)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SP1 Antibody

Binding Specificity: AA 421-470, pThr453

Specificity:

SP1 (Phospho-Thr453) Antibody detects endogenous levels of SP1 only when phosphorylated at Thr453.

PhosphorylationH:T453 M:T455 R:T456

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr453.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.620754, Hs.649191 (NCBI Gene Symbol: SP1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1

Background:

Synonyms: TSFP1, Transcription factor SP1, transcription factor Sp1

NCBI Gene Symbol: SP1

Molecular Weight: 80 kDa

Gene ID: 6667

OMIM: 189906

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction