ERK1/2 antibody (pTyr204)

Catalog No. ABIN1531832

The Rabbit Polyclonal anti-ERK1/2 antibody has been validated for WB, ELISA and IHC. It is suitable to detect ERK1/2 in samples from Human, Mouse and Rat.

Quick Overview for ERK1/2 antibody (pTyr204) (ABIN1531832)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ERK1/2 Antibody

Binding Specificity: AA 170-219, pTyr204

Specificity:

p44/42 MAP Kinase (Phospho-Tyr204) Antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyr204.

PhosphorylationH:Y204/187 M:Y205/185 R:Y205/185

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Tyr204.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.861, Hs.431850 (NCBI Gene Symbol: MAPK3)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: p44/42 MAP Kinase

Background:

Synonyms: ERK-1, ERK1, ERT2, Extracellular signal- regulated kinase 1, Insulin-stimulated MAP2 kinase, kinase ERK1, MAP kinase 1, MAPK 1, MAPK3, Microtubule- associated protein-2 kinase, Mitogen-activated protein kinase 3, MK03, MNK1, p44-ERK1, P44-ERK1, p44-MAPK,

NCBI Gene Symbol: MAPK3

Molecular Weight: 43 kDa

Gene ID: 5595, 5594

OMIM: 601795

UniProt: P27361, P28482