MARCKS antibody (pSer159)

Catalog No. ABIN1531898

The Rabbit Polyclonal anti-MARCKS antibody has been validated for WB and ELISA. It is suitable to detect MARCKS in samples from Human, Mouse and Rat.

Quick Overview for MARCKS antibody (pSer159) (ABIN1531898)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MARCKS Antibody

Binding Specificity: AA 126-175, pSer159

Specificity:

MARCKS (Phospho-Ser158) Antibody detects endogenous levels of MARCKS only when phosphorylated at Ser158.

PhosphorylationH:S159 M:S152 R:S152

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MARCKS around the phosphorylation site of Ser158.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:20000

Comment:

Unigene-Number: Hs.519909, Hs.712721 (NCBI Gene Symbol: MARCKS)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background:

Synonyms: 80K-L protein, MACS, MARCS, myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, protein kinase C substrate, 80 kDa protein, light chain

NCBI Gene Symbol: MARCKS

Molecular Weight: 31 kDa

Gene ID: 4082

OMIM: 177061

UniProt: P29966