TIE1 antibody (Biotin)

Catalog No. ABIN155170

Product Details anti-TIE1 Antibody (Biotin)

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIE1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity: This unconjugated monoclonal antibody will detect native TIE-1 in ELISA experiments and on the surface of different cell types.

Purification: Protein G Chromatography.

Immunogen: Recombinant human soluble TIE-1 protein.

Clone: 6F12

Isotype: IgG1

Application Details

Application Notes: ELISA: 1-15 μg/mL. Western blot: 1-2 μg/mL. FACS: 2-5 μg/mL. Cell sorting: 2-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, BSA (50X)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE1 (TIE1 Products)

Background: The soluble receptor protein consists of the full extracellular domain (Met1-Glu749). The recombinant mature TIE-1/Fc is a disulfide-linked homodimeric protein. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1

Gene ID: 7075

NCBI Accession: NP_005415

UniProt: P35590

Pathways: RTK Signaling