TIE1 antibody (Biotin)

Catalog No. ABIN155170

This Mouse Monoclonal antibody specifically detects TIE1 in WB, EIA and FACS. It exhibits reactivity toward Human.

Quick Overview for TIE1 antibody (Biotin) (ABIN155170)

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIE1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Flow Cytometry (FACS)

Clone: 6F12

Product Details anti-TIE1 Antibody (Biotin)

Specificity: This unconjugated monoclonal antibody will detect native TIE-1 in ELISA experiments and on the surface of different cell types.

Purification: Protein G Chromatography.

Immunogen: Recombinant human soluble TIE-1 protein.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1-15 μg/mL. Western blot: 1-2 μg/mL. FACS: 2-5 μg/mL. Cell sorting: 2-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, BSA (50X)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE1

Background: The soluble receptor protein consists of the full extracellular domain (Met1-Glu749). The recombinant mature TIE-1/Fc is a disulfide-linked homodimeric protein. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1

Gene ID: 7075

NCBI Accession: NP_005415

UniProt: P35590

Pathways: RTK Signaling