TIE1 antibody (AA 435-609)

Catalog No. ABIN7442504

The Rabbit Polyclonal anti-TIE1 antibody is suitable to detect TIE1 in samples from Human. It has been validated for WB and IHC.

Quick Overview for TIE1 antibody (AA 435-609) (ABIN7442504)

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TIE1 Antibody

Binding Specificity: AA 435-609

Purpose: Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

Specificity: The antibody is a rabbit polyclonal antibody raised against Tie1. It has been selected for its ability to recognize Tie1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) corresdonding to Asp435~Gly609 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: Tie1

Background: JTK14, Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1, Tyrosine-protein kinase receptor Tie-1

UniProt: P35590

Pathways: RTK Signaling