TUBB antibody (Tubulin, beta)

Details for Product anti-TUBB Antibody No. ABIN1574254
Antigen
  • M40
  • OK/SW-cl.56
  • TUBB1
  • TUBB5
  • XLOT
  • m40
  • ok/sw-cl.56
  • tubb1
  • tubb5
  • TUBB2A
  • TUBB2B
  • TUBB
  • 1t
  • B1t
  • BETA 56D
  • CG9277
  • DTB2
  • Dmbeta1
  • Dmel\\CG9277
  • T
  • Tub
  • Tubulin
  • beta
  • beta-Tub
  • beta-Tub56D
  • beta-tub
  • beta-tubulin56D
  • beta1
  • beta1-Tubulin
  • beta1-tub
  • beta1Tub
  • beta1t
  • beta1tub
  • beta56D
  • betaTub
  • betaTub1
  • beta[[1]] tubulin
  • beta[[1]]-tubulin
  • betatub(56D)
  • 143391_i_at
  • 3t
  • B3t
  • BETA 60D
  • CG3401
  • D.m.BETA-60D
  • DTB3
  • Dmbeta3
  • Dmel\\CG3401
  • Tub60D
  • beta-Tub60D
  • beta-Tub6D
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3TUB
  • beta3Tub
  • beta3t
  • beta60C
  • betaTub3
  • betaTub60C
  • beta[[3]] tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • betatub60D
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • 2t
  • B2t
  • BETA 85D
  • BETA2
  • CG9359
  • D.m.BETA-85D
  • DTB4
  • Dmbeta2
  • Dmel\\CG9359
  • beta(2)Tu
  • beta(2)Tub
  • beta-Tub85D
  • beta-tub85D
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta85D
  • betaTub2
  • beta[[2]]-tubulin
  • ms(3)KK[D]
  • 4t
  • B4t
  • BETA 98B
  • CG4869
  • DTB1
  • Dmbeta4
  • Dmel\\CG4869
  • beta-Tub97EF
  • beta4
  • beta4t
  • beta97F
  • betaTub4
  • betaTub98BC
  • betaTub98C
  • Tub1
  • bmtub1
  • Tub3
  • bmtub3
  • Tub2
  • bmtub2
  • Tub4
  • bmtub4
  • LOC100101153
  • tubulin beta class I
  • putative cobalt-zinc-cadmium outer membrane resistance protein
  • Tubulin beta chain
  • tubulin beta-2A chain
  • beta-Tubulin at 56D
  • beta-Tubulin at 60D
  • beta-Tubulin at 85D
  • beta-Tubulin at 97EF
  • beta-tubulin
  • beta tubulin
  • tubulin beta class I L homeolog
  • tubulin, beta 5 class I
  • TUBB
  • czcC
  • tbb-6
  • tubb
  • LOC462399
  • betaTub56D
  • betaTub60D
  • betaTub85D
  • betaTub97EF
  • Tub1
  • Tub3
  • Tub2
  • Tub4
  • TVAG_525430
  • TVAG_062880
  • TVAG_456920
  • LOC100101153
  • Tb927.1.2330
  • Tb927.1.2350
  • Tb927.1.2370
  • Tb927.1.2390
  • tubb.L
  • Tubb
  • LOC693049
  • LOC443015
  • Tubb5
Alternatives
anti-General TUBB antibody for Western Blotting
Reactivity
Fish, Mouse (Murine), Rat (Rattus), Rabbit, Pig (Porcine), Cow (Bovine), Goat, Chicken, Human
329
225
210
33
33
27
27
24
18
18
15
15
10
9
8
7
7
6
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
188
184
1
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBB antibody is un-conjugated
11
10
8
8
8
8
8
8
6
3
3
3
3
3
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
282
103
95
79
59
41
34
22
22
8
7
5
3
2
2
1
1
1
Options
Immunogen purified human recombinant full-length β-tubulin protein
Clone 2G7D4
Isotype IgG1
Specificity Beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.
Cross-Reactivity (Details) beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.
Purification Protein A affinity column
Plasmids, Primers & others Plasmids, Primers & others TUBB products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name beta-Tubulin (TUBB Antibody Abstract)
Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (alpha) and beta (beta) chains. beta-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots. beta-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length beta-tubulin protein.
Pathways Microtubule Dynamics, M Phase
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Flow cytometry:1 µg for 1 x 106 cells
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Product cited in: Huang, Nishi, Li, Ho, Dong, Chang, Chang: "Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain." in: The FEBS journal, Vol. 281, Issue 7, pp. 1773-83, 2014 (PubMed).

Sukthankar, Avila, Whitaker, Iwamoto, Morgenstern, Apostolidis, Liu, Hanzlik, Dadachova, Tomich: "Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes." in: Biochimica et biophysica acta, Vol. 1838, Issue 9, pp. 2296-305, 2014 (PubMed).

Infanger, Cho, Lopez, Mohanan, Liu, Gursel, Boockvar, Fischbach: "Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche." in: Cancer research, Vol. 73, Issue 23, pp. 7079-89, 2013 (PubMed).

Wang, Chatterjee, Jeon, Akerman, Vander Heiden, Cantley, Krainer: "Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons." in: Journal of molecular cell biology, Vol. 4, Issue 2, pp. 79-87, 2012 (PubMed).

Did you look for something else?