TUBB antibody (Tubulin, beta)

Details for Product anti-TUBB Antibody No. ABIN1574254
Target
Reactivity
Chicken, Cow, Fish, Goat, Human, Mouse, Pig, Rabbit, Rat
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160
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27
23
23
17
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9
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2
2
2
1
1
1
1
1
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1
1
1
1
1
1
Host
Mouse
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112
1
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBB antibody is un-conjugated
10
10
10
8
7
7
7
7
3
3
3
3
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3
1
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Application
Flow Cytometry (FACS), Western Blotting (WB)
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59
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28
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12
9
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Options
Immunogen purified human recombinant full-length β-tubulin protein
Clone 2G7D4
Isotype IgG1
Specificity Beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.
Cross-Reactivity (Details) beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.
Purification Protein A affinity column
Target
Alternative Name beta-Tubulin (TUBB Antibody Abstract)
Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (alpha) and beta (beta) chains. beta-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots. beta-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length beta-tubulin protein.
Pathways Microtubule Dynamics, M Phase
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Flow cytometry:1 µg for 1 x 106 cells
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Product cited in: Huang, Nishi, Li, Ho, Dong, Chang, Chang: "Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain." in: The FEBS journal, Vol. 281, Issue 7, pp. 1773-83, 2014 (PubMed).

Sukthankar, Avila, Whitaker, Iwamoto, Morgenstern, Apostolidis, Liu, Hanzlik, Dadachova, Tomich: "Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes." in: Biochimica et biophysica acta, Vol. 1838, Issue 9, pp. 2296-305, 2014 (PubMed).

Infanger, Cho, Lopez, Mohanan, Liu, Gursel, Boockvar, Fischbach: "Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche." in: Cancer research, Vol. 73, Issue 23, pp. 7079-89, 2013 (PubMed).

Wang, Chatterjee, Jeon, Akerman, Vander Heiden, Cantley, Krainer: "Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons." in: Journal of molecular cell biology, Vol. 4, Issue 2, pp. 79-87, 2012 (PubMed).