14-3-3 eta antibody

Catalog No. ABIN1580407

This Mouse Monoclonal antibody specifically detects 14-3-3 eta in WB, IF, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for 14-3-3 eta antibody (ABIN1580407)

Target: 14-3-3 eta (YWHAH)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 eta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 3G12

Product Details anti-14-3-3 eta Antibody

Purification: affinity purified antibody

Isotype: IgG1

Application Details

Application Notes: Try at dilutions of 1:500-1:1,000 for immunofluorescence. For western blots try at 1:1,000-1:5,000. A suitable control tissue is rat spinal cord or peripheral nerve homogenate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for 14-3-3 eta

Target: 14-3-3 eta (YWHAH)

Alternative Name: 14-3-3eta

Background: The 14.3.3 family of proteins were originally discovered as prominent protein spots on 2 dimensional gels. They are a family of 28-33 kDa proteins which are often major proteins of the cytoplasm. They function as binding partners for phosphoserine and phosphothreonine sites in other proteins, though they have other binding partners which are not dependent on phosphorylation. These binding interactions are important in the regulation of molecules such as the signaling kinases in the MAP kinase pathway, c-Raf and b-Raf, the proapoptotic molecules Bad and Bax, and the cell cycle regulator Cdc25. There are 7 mammalian 14.3.3 proteins and they are normally expressed as homo or in some cases heterodimers. 14.3.3 eta is widely expressed and concentrated in the nervous system. The HGNC name for 14.3.3 is YWHAH, and an alternate name is tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein 1, due to a role for this protein in activation of tyrosine and tryptophan hydroxylases. One result of this is that the HGNC name is YWHAH. The 14.3.3eta, protein accumulates in the CSF of patients suffering form Creutzfeld Jacob Disease, and can be used for the diagnosis of this disease. Furthermore, this protein binds alpha,-synuclein in the Lewy bodies of Parkinson's disease and has been linked to early-onset schizopherenia. The HGNC name for this protein is YWHAH. ﾠ

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, C21-Steroid Hormone Metabolic Process, Myometrial Relaxation and Contraction