ENO1 antibody

Catalog No. ABIN1580416

This Mouse Monoclonal antibody specifically detects ENO1 in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse, Rat, Cow and Xenopus laevis.

Quick Overview for ENO1 antibody (ABIN1580416)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human, Mouse, Rat, Cow, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 253

Product Details anti-ENO1 Antibody

Purification: affinity purified antibody

Isotype: IgG1

Application Details

Application Notes: Try at dilutions of 1:2,000-1:5,000 for immunofluorescence. For western blots try at 1:5,000-1:10,000. The NNE protein runs at about ~47 kDa on SDS-PAGE gels.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Background: Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. The Enzyme Commission classification number for this class of enzyme is EC4.2.1.11, and they can also be refered to as 2-phospho-D-glycerate hydrolases. There are three enolase proteins each coded for by a distinct gene. They are known as enolase 1, 2 and 3 or alternately as alpha, beta, and gamma,-enolase. Confusingly, although enolase 1 is alpha,-enolase, enolase 2 corresponds to gamma,-enolase while enolase 3 is also known as beta,-enolase. All three enzymes exist as dimers in vivo and the three molecules are very similar in primary amino acids sequence, as can be seen from a sequence alignment downloadable from here. This similarity allows not only homodimers but also most possible heterodimers to form. The expression pattern of the three enzymes differs in a tissue specific manner, so that antibodies to them are useful cell type specific markers, especially as all three molecules are very abundant. Enolase 1 or alpha, is also known as non-neuronal enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer. Enolase 2 or gamma, is also known as neuron specific enolase (NSE), and we also market antibodies directed against this protein, specifically RPCA-NSE. A switch from NNE to NSE occurs in the development of neurons. Enolase 3 or beta, is expressed primarily in muscle cells. Monoclonal antibody MCA-253 was raised against the N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system. The characterization of the antibody has been published in peer reviewed form and in this publication is there referred to as Enol2-53. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1. The HGNC name for this protein is ENO1.