PAB1 antibody

Catalog No. ABIN1580454

This anti-PAB1 antibody is a Mouse Monoclonal antibody detecting PAB1 in ICC, IF, IHC and WB. Suitable for Mammalian and Saccharomyces cerevisiae. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PAB1 antibody (ABIN1580454)

Target: PAB1 (Pab1p (PAB1))

Reactivity: Mammalian, Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: 1G1

Product Details anti-PAB1 Antibody

Purification: conc. tissue culture supernatant

Isotype: IgG2a

Application Details

Application Notes: For immunofluorescence use MCA-1G1 diluted 1/500. For western blots try MCA-1G1 at dilutions of 1/1,000 to 1/5,000.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

References for anti-PAB1 antibody (ABIN1580454)

Reynaud, McGeachy, Noble, Meacham, Ingolia: "Surveying the global landscape of post-transcriptional regulators." in: Nature structural & molecular biology, Vol. 30, Issue 6, pp. 740-752, (2023) (PubMed).

Matia-González, Jabre, Gerber: "Biochemical approach for isolation of polyadenylated RNAs with bound proteins from yeast." in: STAR protocols, Vol. 2, Issue 4, pp. 100929, (2022) (PubMed).

Target Details for PAB1

Target: PAB1 (Pab1p (PAB1))

Alternative Name: Pab1p

Background: Pab1p is a protein which binds poly-A tails of yeast mRNA molecules, and is known as polyadenylate binding protein. It is also referred to as PAB1, ACBP, CST11 and MRNPand the systematic name is YER165w. It is they only yeast poly-A binding protein, and is found in both the cytoplasm and nucleus of yeast cells. The protein has a molecular weight of 64 kDa, and runs on typical SDS-PAGE gels at 67 kDa.