Ubiquitin antibody

Catalog No. ABIN1580462

The Mouse Monoclonal anti-Ubiquitin antibody has been validated for WB, IHC, ICC and IF. It is suitable to detect Ubiquitin in samples from Human, Cow, Chicken, Drosophila melanogaster and C. elegans.

Quick Overview for Ubiquitin antibody (ABIN1580462)

Target: Ubiquitin (Ub)

Reactivity: Human, Cow, Chicken, Drosophila melanogaster, C. elegans

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: Ubi-1-AP

Product Details anti-Ubiquitin Antibody

Purification: affinity purified antibody

Isotype: IgG1

Application Details

Application Notes: MCA-Ubi-1 is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. We recommend a starting dilution of 1:1,000 for this purposes, using ABC or other enzymatic amplification procedures. For immunofluorescence try a dilution of 1:500.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: The antibody is supplied purified form at a concentration of 1 mg/mL. Purification is accomplished using a Protein A affinity column, and the purified antibody is in a phosphate buffered saline solution. We added 11 mM sodium azide preservative .

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C short term or -24 °C long term.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has a important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalantly coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, the Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway