LYVE1 antibody (Biotin)

Catalog No. ABIN1589713

This Rabbit Polyclonal antibody specifically detects LYVE1 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for LYVE1 antibody (Biotin) (ABIN1589713)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYVE1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-LYVE1 Antibody (Biotin)

Specificity: Recombinant human sLYVE-1

Characteristics: Chromosomal location: 11p15
Produced from sera of rabbits immunized with highly pure recombinant human soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1 consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).

Purification: The antibody was purified via an antigen-affinity column and then biotinylated using a standard protocol.

Isotype: IgG

Application Details

Application Notes: FACS: use 3-10 µg/mL

Comment:

Rabbit IG Polyclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Centrifuge vial prior to opening.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Alternative Name: Lyve-1

Background: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule co-localizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Synonyms: LYVE1, HAR, XLKD1, LYVE-1, CRSBP-1

NCBI Accession: NP_006682, NM_006691

UniProt: Q9Y5Y7

Pathways: Glycosaminoglycan Metabolic Process