PODXL antibody

Catalog No. ABIN1589722

This Mouse Monoclonal antibody specifically detects PODXL in IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PODXL antibody (ABIN1589722)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GB6D9

Product Details anti-PODXL Antibody

Specificity: Membrane protein fraction of isolated human glomeruli

Characteristics: Chromosomal location: 7q32-q33
Monoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated human glomeruli as the immunizing antigen.

Purification: The mouse IgG1 antibody (#4D5aE5E6) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Isotype: IgG1

Application Details

Application Notes: IF/IHC: use at 2-5 µg/mL.

Comment:

Monoclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: Podocalyxin

Background: Podocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes. It was shown that endothelial cell membranes contain Podocalyxin. By immunoelectron microscopy Podocalyxin was localized on the luminal membrane domain of endothelial cells, in a patchy distribution. The presence of Podocalyxin was confirmed in SDS extracts of lung tissue by immunoblotting. It is concluded that (a) Podocalyxin is a widespread component of endothelial plasma membranes, (b) it is restricted to the luminal membrane domain, and (c) it is distributed unevenly on the endothelial cell surface.
Synonyms: PODXL, PC, PCLP, Gp200, PCLP-1, Podocalyxin-like protein, GCTM-2 antigen, Gp200

NCBI Accession: NM_001018111, NP_001018121

UniProt: O00592

Pathways: Tube Formation