Endomucin antibody

Catalog No. ABIN1589841

The Rabbit Polyclonal anti-Endomucin antibody has been validated for WB. It is suitable to detect Endomucin in samples from Mouse.

Quick Overview for Endomucin antibody (ABIN1589841)

Target: Endomucin (EMCN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endomucin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Endomucin Antibody

Purpose: Endomucin antibody

Specificity: Recombinant mouse soluble Endomucin

Characteristics: Chromosomal location: 3 G3, 3

Purification: Protein A purified

Immunogen: Recombinant mouse soluble Endomucin (ABIN1589740)

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for Endomucin

Target: Endomucin (EMCN)

Alternative Name: Endomucin

Background: Endomucin-1/2, Mucin-14,Endomucin (endothelial sialomucin, also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60 % and 30 % aa identity with rat and human Endomucin, respectively.

Gene ID: 59308

NCBI Accession: NM_001163522, NP_001156994

UniProt: Q9R0H2