ESM1 antibody

Catalog No. ABIN1589852

The Rabbit Polyclonal anti-ESM1 antibody has been validated for WB and IF. It is suitable to detect ESM1 in samples from Human.

Quick Overview for ESM1 antibody (ABIN1589852)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ESM1 Antibody

Purpose: Endocan/ESM-1 antibody

Specificity: Recombinant human ESM-1

Characteristics: Chromosomal location: 5q11.2

Purification: Protein A purified

Immunogen: Recombinant human ESM-1 (ABIN1589766)

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: 0.5X PBS, pH 7.2

Handling Advice: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for ESM1

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Alternative Name: Endocan/ESM-1

Background: Endocan, ESM1,Endocan, also known as endothelial cell specific molecule1 (ESM1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung. Endocan expression has also been detected in different epithelia and in adipocytes. The expression of endocan is upregulated by TNFα, IL1β, or lipopolysaccharide and downregulated by IFNγ. The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues. The DS chain is covalently attached to serine 137. Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte functionassociated antigen1, LFA1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA1-mediated leukocyte activation. Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo. Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported.

Gene ID: 11082

NCBI Accession: NM_007036, NP_008967

UniProt: Q9NQ30

Pathways: Growth Factor Binding