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Myosin IH antibody (C-Term)

The Goat Polyclonal anti-Myosin IH antibody has been validated for WB, ELISA and IHC. It is suitable to detect Myosin IH in samples from Human and Mouse.
Catalog No. ABIN1590005

Quick Overview for Myosin IH antibody (C-Term) (ABIN1590005)

Target

Myosin IH (MYO1H)

Reactivity

Human, Mouse

Host

  • 15
Goat

Clonality

  • 15
Polyclonal

Conjugate

  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Myosin IH antibody is un-conjugated

Application

  • 15
  • 13
  • 13
  • 3
  • 3
  • 3
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade

Verified
  • Binding Specificity

    C-Term

    Purpose

    Myosin IH

    Sequence

    EDQVYKDKNG QLR

    Cross-Reactivity

    Cow, Human, Mouse, Pig, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 110 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118 kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3 μg/mL. An additional band of unknown identity was also consistently observed at 28 kDa. This

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    Myosin IH (MYO1H)

    Alternative Name

    Myo1H

    Background

    MYO1H, myosin IH, myosin-1H, myosin-Ih, unconventional myosin-Ih

    Gene ID

    283446, 231646, 687866

    NCBI Accession

    NP_001158045
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