HDAC1 antibody (Internal Region)

Catalog No. ABIN1607883

Supplier Product No.: 600-401-j16

This anti-HDAC1 antibody is a Rabbit Polyclonal antibody detecting HDAC1 in WB, ELISA, IHC and FM. Suitable for Human, Mouse, Rat and Primate.

Quick Overview for HDAC1 antibody (Internal Region) (ABIN1607883)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human, Mouse, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-HDAC1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-j16

Supplier: Rockland

Purpose: HDAC1 (near C-terminus) Antibody

Cross-Reactivity (Details): This antibody is specific towards HDAC1.

Characteristics: Synonyms: rabbit anti-HDAC-1 antibody, HDAC1, HDAC 1, HD 1 antibody, histone deacetylase 1 antibody, Histone deacetylase-1, HD1 antibody, RPD3L1, reduced potassium dependency yeast homolog like 1 antibody

Purification: Anti-HDAC1 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: HDAC1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human HDAC1.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-HDAC1 antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.59 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background: Background: HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. Anti-HDAC1 antibodies are ideal for researchers interested in Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, Checkpoint signaling, Core ESC Like Genes, Chromatin Research, DNA Double Strand Break Repair, Epigenetics, DNA Repair, Histone Deacetylases, and Stem Cell Marker research.

Gene ID: 3065

NCBI Accession: NP_004955

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis