PTRF antibody (C-Term)

Catalog No. ABIN1607897

Supplier Product No.: 600-401-j30

Product Details anti-PTRF Antibody

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-j30

Supplier: Rockland

Purpose: PTRF Antibody

Cross-Reactivity (Details): This antibody is specific towards Cavin 1 PTRF.

Characteristics: Synonyms: rabbit anti-Cavin1 antibody, rabbit anti-PTRF antibody, CAVIN 1, cavin-1, Cavin1, polymerase I and transcript release factor, Caveolae-associated protein 1

Purification: Anti-PTRF was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: PTRF affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human PTRF.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-PTRF antibody is useful for ELISA, Immunostaining, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Protein Molecular weight: 60 kDa. Expect a band approximately ~40.5 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for PTRF

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Alternative Name: CAVIN1 (PTRF Products)

Background: Background: PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13. Anti-PTRF antibodies are ideal for researchers interested in Diabetes Research, Lipid and Metabolism research.

Gene ID: 284119

NCBI Accession: NP_036364

UniProt: Q6NZI2